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Fluorescent Lightsheet Microscopy

Introduction

The facility is set up to generate artifact-free 3D representations of intact specimens (e.g. organs, biopsy samples or  organoids .) Preserving the 3D structure of tissues and organs will boost our understanding of how they work and will provide valuable insight into pathogenic processes.

The facility has a lot of experience with whole-mount staining of different tissues with different antibodies. Following immunostaining, clearing techniques (e.g. 3DISCO) are used to make tissues transparent. The cleared samples can then be imaged with an Ultramicroscope II (Lightsheet microscope) systems. These systems can image both water-based and organic solvent cleared samples. We generally use 3DISCO or iDISCO for tissue clearing, but the facility is set up to use other clearing methods as well (e.g. CLARITY).

For visualization and analysis of microscopic data we use Imaris software as well as open source software (e.g. FIJI and ClearMap). If you have questions or would like to discuss a potential project, please use the application form.