| General | ||
| Type | Light sheet microscope | |
| Name | UltraMicroscope II | |
| Company | LaVision BioTec GmbH |
| Sheet optics | ||
| Illumination | Uni- & bidirectional | |
| Numerical aperture | 0.0148 – 0.148 | |
| Thickness | 4 µm – 24 µm | |
| Focus positioning | Dynamic | |
| Refractive index matching | 1.33 – 1.56 | |
| Zoom optics | ||
| Zoom Body type | Olympus MVX-10 | |
| Zoom ratio | 0.63x – 6.3x (1:10) | |
| Detection optics | ||
| Objective lens | Olympus MVPLAPO 2x | |
| Numerical aperture | 0.07 – 0.5* | |
| FOV diagonal | 1.7 – 17.6mm | |
| Effective Magnification (lens + dipping cap + zoom) | Standard Dipping Cap: 1.26x – 12.6x
Correction Dipping Cap: 1.35x-13.5x |
|
| Working distance | Standard Dipping Cap: 10mm
Correction Dipping Cap: 5.7mm |
|
| Chromatic correction | Dynamic 400 nm – 850 nm | |
| Detector | ||
| Type | sCMOS | |
| Name | Neo 5.5 sCMOS | |
| Company | Andor | |
| Pixel | 2560 x 2160 | |
| Pixel size | 6.5 µm x 6.5 µm | |
| Maximum frame rate | 100 fps @ full frame | |
| Read noise | 1e | |
| Imaging chamber |
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| Imaging solution | Default = DBE
but also aqueous buffers are possible |
|
| Light source | ||
| Laser module | ||
| Emission filters |
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| Microscope software | ||
| Imspector | Version: 7.1 |
Further information:
| *Zoom factor and corresponding NA and resolution by pixel size (with standard and Correction Dipping Cap (CDC)) |