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Protocols

Tissue Clearing:

Microscope settings:

Validated antibodies:

Antigen Host Vendor Catalog Dilution Type of staining
Calbindin Rabbit Swant CB -38a 1:15000 3DISCO
Cfos Rabbit Synaptic Systems 226003 1:500 iDISCO
CHX10 (VSX2)
Sheep Abcam ab16141 1:500 3DISCO
CTIP2 Rat Abcam ab18465 1:100 iDISCO
GFP Rabbit Invitrogen A11122 1:1000 3DISCO 
Islet1 Goat Neuromics GT15051 1:2000  3DISCO
L1 Rat Milipore MAB5272 1:500 3DISCO
Netrin Rabbit Abcam ab126729 1:500 3DISCO 
Nrp2 Goat R&D AF567 1:1000 3DISCO
Pax6 mouse DSHB pax6 1:200 iDISCO
pHH3 Rabbit Cell Signaling 9701  1:800 3DISCO 
RFP Rabbit Rockland 600-401-379 1:1000 iDISCO
Robo1 Goat R&D AF1749 1:300 3DISCO
Robo3 Goat R&D AF3155 1:300 3DISCO
Satb2 Rabbit Abcam ab34735 1:200 iDISCO
Six3 Rabbit Rockland 600-401-A26 1:500 3DISCO
TH Sheep Pelfreez P60101-150 1:500 3DISCO 
TH Rabbit Millipore AB152 1:1000 3DISCO  / iDISCO
TrkA Goat R&D AF1056 1:400   3DISCO
TrkC Goat R&D AF1404 1:400  3DISCO