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Neural iPSC/Genome Editing

Introduction

The MIND iPSC facility was set up to provide researchers with the opportunity to generate and work with human (patient-derived) induced Pluripotent Stem Cells (iPSC). In the past years, many different iPSC lines have been generated to develop specific models for studying neural development, physiology and disease (publications). The possibility of generating patient-derived iPSC lines followed by differentiation into desired neural cell types has the promise to provide new, groundbreaking insights into disease pathology, genetic etiologies and to facilitate the development of therapeutic approaches.

The facility has expertise with generating iPSC from different cell types (e.g. skin fibroblasts, PBMCs) using different strategies (e.g. lentiviral, Sendai virus), their characterization at the molecular and cellular level and differentiation into various neuronal cell types (e.g. motor and sensory neurons, cortical neurons), neural cell types (e.g. astrocytes) or other relevant cell types (e.g. muscle cells).

Further, CRISPR/Cas strategies have been implemented for genome editing of iPSC, ranging from the insertion of specific mutations to insertion of for example marker genes. Part of these projects are conducted in collaboration with external partners.

If you have questions or would like to discuss a potential project, please use the application form.